FOOD BEVERAGE BIOTECHNOLOGY
Food and beverage fermentations are traditional and widely used processes that involve the conversion of raw materials into various products through the action of microorganisms. Microbial fermentation is a natural and controlled process that imparts specific flavors, textures, and preservation qualities to foods and beverages.
Here are some common examples of food and beverage fermentations:
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Bread Fermentation:
- Process: Baker's yeast (Saccharomyces cerevisiae) is used to ferment the sugars in flour, producing carbon dioxide gas that causes the dough to rise. This process results in the leavening of the bread, creating a light and fluffy texture.
- Product: Bread, rolls, and other baked goods.
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Yogurt Fermentation:
- Process: Lactic acid bacteria, such as Lactobacillus bulgaricus and Streptococcus thermophilus, ferment milk sugars (lactose) to produce lactic acid. This acidification thickens the milk and gives yogurt its characteristic tangy flavor.
- Product: Yogurt and various cultured dairy products.
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Cheese Fermentation:
- Process: Lactic acid bacteria and specific molds are used to ferment milk and curdle it. The curds are then separated from the whey, and the cheese is aged to develop its flavor and texture.
- Product: Various types of cheeses, such as cheddar, brie, and blue cheese.
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Beer Fermentation:
- Process: Yeast, typically Saccharomyces cerevisiae, ferments sugars derived from malted grains, producing alcohol and carbonation. Hops are often added for flavor and aroma.
- Product: Beer with a range of styles and flavors.
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Wine Fermentation:
- Process: Yeast ferments the sugars in grapes, converting them into alcohol and carbon dioxide. The process may involve red or white winemaking techniques, and the fermentation can take place with or without grape skins.
- Product: Red wine, white wine, and various grape-derived beverages.
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Sauerkraut Fermentation:
- Process: Lactic acid bacteria naturally present on cabbage ferment the sugars, producing lactic acid. This process leads to the sour taste and preservation of the cabbage.
- Product: Sauerkraut, a fermented cabbage dish.
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Kimchi Fermentation:
- Process: Similar to sauerkraut, but with the addition of various spices and seasonings. Lactic acid bacteria and other microorganisms ferment the vegetables.
- Product: Kimchi, a traditional Korean fermented vegetable dish.
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Sourdough Fermentation:
- Process: Wild yeast and lactic acid bacteria from the environment ferment a mixture of flour and water, creating a natural leaven. This leaven is used to raise bread without commercial yeast.
- Product: Sourdough bread with a distinctive tangy flavor.
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Vinegar Fermentation:
- Process: Acetic acid bacteria convert ethanol (alcohol) into acetic acid, resulting in the sour taste of vinegar.
- Product: Various types of vinegar, such as apple cider vinegar and balsamic vinegar
Below is a table summarizing the roles of microorganisms as food and their involvement in food processing, along with some key enzymes used in food processing:
Microorganisms as Food | Examples |
---|---|
Bacteria | Yogurt and fermented dairy products |
Fungi | Edible mushrooms, fermented soy products |
Algae | Spirulina, Chlorella (used in supplements) |
Yeast | Baker's yeast, Brewer's yeast |
Enzymes and Food Processing | Role in Food Processing | Examples of Use |
---|---|---|
Proteases | Meat tenderization | Papain (from papaya) for tenderizing meat |
Amylases | Starch breakdown in baking | Used in bread making, brewing for starch-to-sugar conversion |
Lipases | Fat breakdown in cheese production | Used in cheese-making for flavor development |
Cellulases | Plant cell wall breakdown for juice extraction | Used in fruit juice production |
Pectinases | Pectin breakdown in fruit processing | Used in fruit juice extraction and clarification |
Transglutaminase | Binding proteins in meat products | Improves texture and appearance of processed meat products |
Lactase | Lactose breakdown for lactose-free products | Used to make dairy products suitable for lactose-intolerant individuals |
Catalase | Decomposing hydrogen peroxide in baking | Improves quality in the bread-making process |
Papain and Bromelain | Meat tenderization | Proteolytic enzymes used to tenderize meat |
Glucose Isomerase | Conversion of glucose to fructose | Used in the production of high-fructose corn syrup |
Category | Subcategory | Examples | Role/Function | Sources |
---|---|---|---|---|
Amino Acids | Essential Amino Acids | Leucine, Isoleucine, Valine | Crucial for protein synthesis and physiological functions. | Meat, dairy, eggs, quinoa, soy. |
Non-Essential Amino Acids | Alanine, Glutamine, Glycine | Synthesized by the body; contribute to protein synthesis and metabolic processes. | Meat, dairy, grains, legumes. | |
Branched-Chain Amino Acids (BCAAs) | Leucine, Isoleucine, Valine | Play a role in muscle protein synthesis and energy production. | Meat, dairy, legumes. | |
Conditionally Essential AAs | Arginine, Tyrosine, Cysteine | Typically non-essential but become essential under certain conditions (e.g., illness). | Obtained from a balanced diet; synthesized by the body normally. | |
Vitamins | Fat-Soluble Vitamins | A, D, E, K | Essential for various functions including vision, bone health, and antioxidant activity. | Liver, fish oils, dairy, fruits, vegetables. |
Water-Soluble Vitamins | C, B1, B2, B3, B5, B6, B7, B9, B12 | Roles include antioxidant activity, energy metabolism, and nerve function. | Fruits, vegetables, whole grains, meat, dairy. | |
Sweeteners | Natural Sweeteners | Sucrose (Table Sugar), Honey, Maple Syrup | Naturally occurring sweeteners used in various foods and beverages. | Sugar cane, sugar beet, bees, sugar maple trees. |
Artificial Sweeteners | Aspartame, Saccharin, Sucralose | Low-calorie or non-caloric sweeteners used in sugar-free and diet products. | Manufactured; often used in processed foods and beverages. | |
Sugar Alcohols | Xylitol, Sorbitol, Erythritol | Sugar substitutes with fewer calories, used in sugar-free products. | Naturally occurring in small amounts in certain fruits, vegetables. |
Organic Acids:
Type | Examples | Sources | Role/Function |
---|---|---|---|
Citric Acid | Citrus fruits, tomatoes | Fruits, vegetables, especially citrus fruits. | Flavor enhancer, acidity regulator in food and beverages. |
Lactic Acid | Fermented dairy, pickles | Fermented foods, pickled vegetables, dairy products. | Acidifier, preservative, contributes to the tangy flavor in fermented foods. |
Acetic Acid | Vinegar | Vinegar, especially in fermented products. | Flavoring agent, food preservation. |
Malic Acid | Apples, cherries | Apples, cherries, some berries. | Tart flavor in fruits, used as a food additive. |
Tartaric Acid | Grapes | Grapes, certain fruits, especially in wine. | Acidulant in food and beverages, especially in winemaking. |
Sorbic Acid | Berries, herbs | Berries, herbs, used as a preservative in food products. | Food preservative, inhibits the growth of molds and yeasts. |
Polysaccharides:
Type | Examples | Sources | Role/Function |
---|---|---|---|
Starch | Cereals, potatoes, legumes | Cereals, tubers, legumes. | Major carbohydrate storage in plants, used as a thickening agent in food. |
Cellulose | Plant cell walls | Fruits, vegetables, grains. | Structural component in plant cell walls, dietary fiber. |
Chitin | Exoskeletons of crustaceans, insects | Shrimp shells, insect exoskeletons. | Structural component in the exoskeletons of arthropods. |
Glycogen | Animal liver, muscles | Liver, muscles in animals. | Carbohydrate storage in animals, especially in the liver and muscles. |
Hyaluronic Acid | Connective tissues, skin | Animal connective tissues, synovial fluid. | Lubrication in joints, skincare products. |
Pectin | Fruits, especially apples and citrus fruits | Apples, citrus fruits, some vegetables. | Gelling agent in jams and jellies, used in food processing. |